Study of Polynucleotide Phosphorylase (PNPase) Extracted by Lysozyme-Ultrasonication
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Graphical Abstract
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Abstract
PNPase was extracted by lysozyme-ultrasonication from E. coli. In order to determine the nature and quantity of PNPase, PAGE, activity determination of PNPase and enzymatic synthesis of poly I were carried out. The results indicated that PNPase extracted
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