Determination of Fructose and Glucose in Invert Sugar Injection by HPLC

OBJECTIVE To establish a method for the determination of fructose and glucose in invert sugar injection. METHODS Hamilton HC-75H(305 mm×7.8 mm, 9 μm) was used. The mobile phase was 0.04 mol·L^-1 phosphoric acid at a flow rate of 0.5 mL·min^-1. The determination wavelength was 200 nm. The temperature of the column was 50 ℃. RESULTS To fructose and glucose, the limit of detection was 0.28 μg and 0.52 μg, the linear range was 1.27-3.81 mg·mL^-1(r=0.999 4) and 1.26-3.76 mg·mL^-1(r=0.999 2), the spiked recovery was 100.8% and 100.4%, respectively. CONCLUSION The method is suitable for the determination of fructose and glucose in invert sugar injection.