Inhibitory Effect of Water Extracts from Helicteres Angustifolia on HBsAg and HBeAg in HepG2.2.15 Cells

OBJECTIVE To observe the effect of water extracts from Helicteres angustifolia (WHA) on excreting HBsAg and HBeAg of HepG2.2.15 cells. METHODS The toxicity of WHA on HepG2.2.15 cells was detected by means of MTT. The inhibitory effects of WHA on HepG2.2.15 cells excreting HBsAg and HBeAg were evaluated with method of enzyme-linked immunosorbent assay(ELISA). RESULTS　The half toxic concentration (TC50) of WHA on HepG2.2.15 cells was 482.1 mg·L^-1. The half inhibitory concentration (IC50) of WHA on HBsAg was 7.3 mg·L^-1, the trealment index (TI) of WHA on HBsAg was 66, and the IC50 of WHA on HBeAg was 14.6 mg·L^-1, TI was 33. CONCLUSION WHA can significantly inhibit HBV in vitro, and is a lower toxic drug.