Determination of Four Components in Sanhuang Decoction by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of four important active components in Sanhuang decoction. METHODS An Agilent C₁₈ column (4.6 mm×200 mm, 5 μm) was used with the mobile phase of 1% acetic acid methanol solution-1% acetic acid by gradient dilution, at the flow rate of 1.0 mL·min^-1 and detection wavelength of 329 nm. RESULTS The linear calibration curves of chlorogenic acid, jatrorrhizine hydrochloride, berberine hydrochloride and palmatine hydrochloride were obtained in the ranges of 0.5-4.0 μg·mL^-1(r=0.999 1), 0.5-4.0 μg·mL^-1 (r=0.999 7), 0.5-12.0 μg·mL^-1(r=0.999 3), 0.3-4.0 μg·mL^-1 (r=0.999 9), respectively. Their average recoveries (n=3) for the assay were 99.0%(RSD=1.13%)，99.3%(RSD=1.20%)，99.0%(RSD=0.27%) and 99.8%(RSD=0.88%), respectively. The intra- and inter-day RSDs were all less than 2.0%. CONCLUSION This method is accurate, sensitive and suitable for quality control of Sanhuang decoction.