Optimization of Extraction of Crocin from Crocus Sativus L. by Orthogonal Regression Design

OBJECTIVE To study the optimal extraction condition of crocin from Crocus sativus L. METHODS Taking the total extraction ratio of crocin from Crocus sativus L. as an index, the quadratic orthogonal regression design which was based on the single factor method including the concentration of ethanol, extraction time and extraction temperature was employed to build the equation and to optimize the extraction technique. RESULTS The optimized condition was as follows: the concentration of ethanol was 0.52%, extracted 30 minutes at the temperature of 30 ℃, and the extraction ratio reached 0.643 5 mg·g^-1. CONCLUSION Under the optimal extraction condition, the total extraction ratio of crocin from Crocus sativus L. is much higher.