Determination of Rhaponiticin Illegally Added in Fule Granule

OBJECTIVE To establish a method for qualitative determination of illegal composition of rhaponiticin in Fule granules. METHODS TLC and LC-MS were employed to isolate and analyze the extract from Fule granule suspected of illegal rhaponiticin and rhaponiticin was identified by HPLC-DAD. RESULTS The Fule granule was detected to contain illegal composition of rhaponiticin. CONCLUSION The established method is specific, sensitive, convenient, and can be used efficiently for control and surveillance of illegal composition of rhaponiticin.