Comparison of Ultrasonic Method and Smashing Tissue Extraction Process of Gentiopicroside from Gentianae Radix et Rhizoma

OBJECTIVE To compare the ultrasonic extraction and smashing tissue extraction process of gentiopicroside from Gentianae Radix et Rhizoma. METHODS An HPLC method was applied to determine the content of gentiopicroside from Gentianae Radix et Rhizoma. The best extraction technology was optimized by L₉(3⁴) orthogonal test with the concentration of ethanol, extraction time, ultrasonic power, material-liquid ratio as factors for ultrasonic extraction and with the concentration of ethanol, material-liquid ratio, extraction time as factors for smashing tissue extraction process, the extraction rate of gentiopicroside was chosen as index. RESULTS Smashing tissue extraction process was superior to ultrasonic extraction, optimal extraction technology were as follows: extraction solvent of 50% ethanol, the material-liquid ratio of 1∶60, extracting time of 3 min. CONCLUSION The smashing tissue extraction process is simple and economical with high extraction rate and can provide parameters for exploitation and utilization of Gentianae Radix et Rhizoma.