Determination of Polysorbate 80 in the Zedoary Turmeric Oil Injection by HPLC-ELSD

OBJECTIVE To develop an HPLC-ELSD method for the determination of polysorbate 80 in the Zedoary turmeric oil injection. METHODS The column was TSK-GEL G2000 SWXL(7.8 mm×300 mm, 5 μm). The mobile phase was 20 mmol·L^-1 ammonium acetate-acetonitrile (90∶10) at the flow rate of 0.6 mL·min^-1. The drift tube temperature was 103 ℃, and the gas flow rate of nitrogen was 2.3 L·min^-1. RESULTS The polysorbate 80 has good linearity in the range of 2.259-28.235 μg(r=0.997 8). The average recovery was 102.5%(n=6, RSD=1.73%), repeatability experiments showed that RSD(n=6) was 0.60%. CONCLUSION The method is rapid, accurate, stable, and can be used to determinate polysorbate 80 in the Zedoary turmeric oil injection.