Determination of Linezolid Content and Related Substances in Linezolid Injection by HPLC

OBJECTIVE To establish an HPLC method for the determination of linezolid and related substances in linezolid injection. METHODS The analytical column was Eclipse XDB-C₁₈, the mobile phase was methanol-water(45∶55), the flow rate was 1.0 mL·min^-1. The detection wavelength was 254 nm, and the column temperature was 25 ℃. RESULTS The linear range of calibration curve was 0.5-250 mg·L^-1(r=0.999 8), and the limit of detection was 1 ng. The average recovery of three concentrations were 99.3%, 100.9%, 100.5%, respectively. CONCLUSION The method is simple, sensitive and accurate, and can control the quality of the preparation effectively.