Study on Extraction of Active Constituents of Yandeng Dripping Pills with Macroporous Adsorption Resin

OBJECTIVE To establish the separation and enrichment technology for active constituents in Yandeng dripping pills with macroporous adsorption resin. METHODS The adsorption capacity and elution ratio of scutellarin were used as evaluating criteria. The static adsorption and separation were used in investigation of macroporous adsorption resin; the dynamic adsorption and separation were used in studying the condition of adsorption and separation. RESULTS AB-8 had better property of separation and purification and the optimal technological conditions were: the concentration of the sample of Semen deseuraniae was 8 mg·mL^-1; the flow velocity of adsorption was 5 mL·min^-1; washing water was 13 times of resin volume; the eluting reagent was 60% ethanol(2 times of the resin volume); eluting velocity was 1 mL·min^-1. CONCLUSION The technology of separating and purifying active constituents in Yandeng dripping pills with AB-8 is stable and effective, and can be applied to manufacture.