Determine of Gossypol in Cottonseed Soapstock by HPLC

OBJECTIVE To determine the content of total gossypol and liberation gossypol by HPLC. METHODS The analysis was performed on a Waters Bondapak C₁₈ Column(3.9 mm×150 mm, 4 μm) at 25 ℃ with acetonitrile-0.7% phosphoric acid water solution(80∶20) as the mobile phase and the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 238 nm. RESULTS Gossypol have good linearity in the range of 10-60 μg·mL^-1(r=0.999 4). The average recovery was 99.03%, RSD=1.72%(n=3). CONCLUSION The developed method is convenient, rapid and accurate with high repeatability, which is suitable to assay the quality of gossypol.