Determination of Notoginsenoside R₁, Ginsenoside Rg1 in Xinkeshu Capsule by HPLC-ELSD

OBJECTIVE To determine the content of notoginsenoside R₁, ginsenoside Rg1 in Xinkeshu capsule. METHODS The notoginsenoside R₁, ginsenoside Rg1 in Xinkeshu capsule were separated on a COSMOSIL 5C¹⁸-PAQ Packed column(250 mm×4.6 mm, 5 mm). The mobile phase was acetonitrile-water(27∶73) at the flow rate of 1 mL·min^-1. The analytes were detected using ELSD. The drift tube temperature was 41 ℃, and gas press was 350 kPa. RESULTS The responses of notoginsenoside R₁, ginsenoside Rg1 were linear in the ranges of 0.123 2-6.16 μg and 0.327 2-16.36 μg, respectively. The average recoveries were 98.7% and 99.2% with RSD 2.72% and 1.54%, respectively. CONCLUSION The described method is simple and accurate. It can be used for the quality control of Xinkeshu capsule.