Determination of 2,3,5,4′-tetrahydroxy-tilbene-2-O-β-D-glucoside in Shouwugan Preparation and Its Tissue Distribution in Mouse

OBJECTIVE To establish a method for the determination of 2,3,5,4′-tetrahydroxy-tilbene-2-O-β- D-glucoside (TSG) in Shouwugan preparation, and to study the tissue distribution of TSG in mouse. METHODS Analyses was performed at 25 ℃ on Agilent XDB C₁₈(150 mm×4.6 mm, 5 μm) column with acetonitrile-water (20∶80) as mobile phase at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 320 nm. RP-HPLC method was developed for determination of TSG in organs of mouse. RESULTS Linearity of TSG was good at 0.053-0.489 μg. The recovery of TSG at low, middle and high concentration was (101.6±1.3)%, (100.9±2.3)%, (100.8±2.4)%, respectively. The TSG was distributed on liver mostly, the following was lung, kidney, heart and so on. CONCLUSION The method is simple and accurate, which can be used for quality control of Shouwugan preparation. The TSG is distributed on many organs, especially on liver, and it can affect brain stem by passing throw blood-brain barrier.