Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC

OBJECTIVE To establish a high performance liquid chromatographic method for the determination of phebalosin in Murrayae Folium et Cacumen. METHODS The analysis was carried out on an Agilent Esclipe XDB-C18 column(150 mm×4.6 mm, 5 μm), using a mixture of 55% methanol and 45% water as the mobile phase at a flow rate of 1 mL·min^-1 at 25 ℃, the wavelength for measurement was 332 nm. RESULTS The calibration curve was linear in the range of 20.72-207.2 μg·mL^-1(r=0.999 8); the average recovery was 98.41% with RSD of 1.65%(n=6). CONCLUSION The method is simple, accurate and reproducible, and it is suitable for the determination of phebalosin in Murrayae Folium et Cacumen.