Effect of Matrine on VEGF mRNA and Protein Expression Levels of Lewis Lung Carcinoma in C57BL/6J Mouse

OBJECTIVE To investigate the effect of matrine on Lewis lung carcinoma in C57BL/6J mouse and to explore its molecurlar mechanism. METHODS The model of mouse with Lewis lung carcinoma was made, the experimental mice were randomly divided into normal saline group, matrine groups at varied concentrations and cytoxan group. Tumor inhibitory rate and microvessel density (MVD) were measured; the mRNA and protein expression of VEGF were determined with RT-PCR and Western blot. RESULTS There were significant differences from inhibitory rates of primary tumor and MVD between matrine groups and normal saline group(P<0.05 or P<0.01); VEGF mRNA and protein expression levels in matrine groups were significantly lower than in normal saline group. CONCLUSION Matrine could inhibit the growth of implanted Lewis lung carcinoma in C57BL/6J mouse at different levels and reduce MVD of Lewis lung carcinoma obviously, which was probably because that matrine reduced the expressions of VEGF mRNA and protein of Lewis lung carcinoma.