The Extract and the Determination of Ginsenoside Rg₁ and Rb₁ of Total Saponins of Stem Base of Panax Notognseng

OBJECTIVE To establish an LC-MS/MS method for simultaneous determination of ginsenosides Rg₁ and Rb₁ in total saponins of stem base of Panax notognseng. METHODS BDS HYPERSUL C₁₈ (150 mm×2.1 mm, 5 mm) was used with a mobile phase of acetonitrile-water(gradient elution). The column temperature was 30 ℃. The ginsenosides Rg₁ and Rb₁ were detected by the negative electrospray ionization mode (Rg₁ m/z 799.6→475.5; Rb₁ m/z 1107.9→783.7; paclitaxel m/z 852.5→525.3). RESULTS The calibration curves of ginsenosides Rg₁ and Rb₁ were linear in the range of 0.173-17.3 mg·mL^-1 and 0.159-16.0 mg·mL^-1 respectively. The precision and accuracy were accord with the requirements. CONCLUSION The results show that this method established in the present research is accurate, sensitive and specific. And it is suitable for simultaneous determination of ginsenosides Rg₁ and ginsenosides Rb₁ in total saponins of stem base of Panax notognseng.