Study on Extraction of Total Polysaccharides in Chrysanthemi Indici Flos

OBJECTIVE To study on extraction of total polysaccharides in Chrysanthemi Indici Flos. And determine total polysaccharides in water extraction of Chrysanthemi Indici Flos from various habitats. METHODS Total polysaccharides were determined with phenol-sulfuric acid method and orthogonal test was used to select the best coloration. RESULTS Recovery of total polysaccharides was 100.3%, RSD was 1.19%, there was difference among Chrysanthemi Indici Flos from various habitats. CONCLUSION The method is accurate, repeatable and suitable to determine total polysaccharides in water extraction of Chrysanthemi Indici Flos.