Preparation of Flexible Nano-liposomes Co-loaded with Trans-retinoic Acid and Betamethasone and Its Effect on Abnormal Proliferation in Psoriatic Cell Model

OBJECTIVE To investigate the synergistic therapeutic effect of flexible nano-liposomes co-loaded with trans-retinoic acid and betamethasone(TRA-BT-FNL) in psoriasis cell model, and to provide a theoretical basis for the development of TRA-BT-FNL.

METHODS Used fluorescence labeling technology(ODA-FITC tracing method) to systematic evaluate cellular uptake efficiency and transdermal drug delivery performance of flexible nano-liposomes; used keratinocyte growth stimulating factor(KGF) to induce HaCaT cells to establish a psoriasis abnormal proliferation cell model; used MTT method with OD value as an indicator to investigate the effect of TRA-BT-FNL on the abnormal proliferation of cell models; enzyme-linked immunoassay(ELISA) was used to detect the changes of inflammatory factors.

RESULTS The uptake of flexible nano-liposomes on HaCaT cells was time-dependent and had good transdermal permeability. KGF stimulated HaCaT cells for 24 h, and the concentration of 80 ng·mL⁻¹ could establish a model of abnormal proliferation of psoriasis. TRA-BT-FNL could significantly inhibit the abnormal proliferation of HaCaT psoriasis cell model, and ELISA showed that it could significantly reduce the secretion of TNF-α and IL-6. All the results showed that the combination of two drugs was superior to the single drug group, and the flexible nano-liposome group was superior to the free drug group.

CONCLUSION TRA-BT-FNL can be ingestioned by keratinocyte better, which has good percutaneous permeability, it can inhibit the abnormal proliferation of keratinocytes and expression of the inflammatory factors such as TNF-α, IL-6, which play an important role in the treatment of psoriasis.