Growth Modelling of Burkholderia Cepacia Complex

OBJECTIVE  To investigate the effects of nutrient concentration and temperature on growth kinetics of type strains in the Burkholderia cepacia complex(Bcc), and predict their proliferation risks.

METHODS  Growth kinetic parameters under different nutrient conditions and temperature regimes were estimated using Baranyi and primary models, and predictive microbiology methods were employed to evaluate the proliferation risks.

RESULTS Nutrient concentration positively correlated with maximum population densityTSB: 9.80−10.08 lg(CFU·mL⁻¹); 1/100 TSB: 9.01−9.21 lg(CFU·mL⁻¹), whereas maximum specific growth rate(μ_max) and lag phase duration showed no significant variation, demonstrating Bcc's oligotrophic tolerance. Temperature critically regulated growth kinetics within 20−40 °C, B. multivorans exhibited higher μ_max and lower minimum growth temperature compared to B. cepacia and B. cenocepacia.

CONCLUSION  This study provides essential kinetic parameters for predicting Bcc proliferation risks in pharmaceutical manufacturing and storage, offering critical evidence for optimizing contamination control strategies.