OBJECTIVE To establish the fingerprint profiles of aqueous Succus Bambusae extract from different species of the same genus, explore the anti-inflammatory activity-spectrum relationship, and screen for key components with anti-pharyngitis properties.
METHODS HPLC was employed to establish the fingerprint profiles of 15 batches of bamboo extract from different species. An lipopolysaccharide-induced inflammation model in RAW 264.7 cells was used, with NO inhibition rate as an indicator. Spectrum-effect relationships were constructed using grey correlation analysis and partial least squares regression. The therapeutic efficacy of p-coumaric acid in the treatment of acute pharyngitis inrats was confirmed using the component knockout method, resulting in a significant reduction in serum levels of IL-1β, IL-6, and TNF-α.
RESULTS The fingerprint profiles of aqueous Succus Bambusae extract revealed 15 common peaks, with p-coumaric acid and(+)-liriodendrin-9’-O-glucoside identified by comparison with reference standards. Spectral-activity relationship analysis indicated that chromatographic peaks 7, 8, and 9(coumaric acid), as well as peak 10(+)-Lyoniresinol 9'-O-glucoside, might be critical contributors to the anti-inflammatory activity of aqueous Succus Bambusae extract. It was verified that p-coumaric acid can significantly reduce the levels of IL-1β, IL-6 and TNF-α in serum of rats with acute pharyngitis, and is an important anti-pharyngitis component.
CONCLUSION The established fingerprint profile can be used to evaluate the quality of aqueous Succus Bambusae extract. The spectrum-effect relationship study preliminarily elucidated the anti-inflammatory active substances of aqueous Succus Bambusae extract, and component knockout experiments identified p-coumaric acid as one of the key components for its anti-pharyngitis effect, providing a reference for quality control and clinical application of aqueous Succus Bambusae extract.
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