Study on the Anti-Oxidant Stress Injury Activity of Salviae Miltiorrhizae Radix Et Rhizoma-Carthami Flos-Notoginseng Radix et Rhizoma Combination based on the D-galactose Induced Oxidative Stress Mice Model

OBJECTIVE  To investigate the anti-oxidative stress injury effects of the Salviae Miltiorrhizae Radix Et Rhizoma-Carthami Flos-Notoginseng Radix et Rhizoma combination based on the D-galactose induced oxidative stress mice model.

METHODS  The chemical compositional of the Salviae Miltiorrhizae Radix Et Rhizoma-Carthami Flos-Notoginseng Radix et Rhizoma compatible sample was identified by UPLC-QTOF-MS. A mice model of oxidative stress was established by intrabitoneal injection of D-galactose, and the effects of Salviae Miltiorrhizae Radix Et Rhizoma, Carthami Flos, Notoginseng Radix et Rhizoma on oxidative stress injury were evaluated. The contents of superoxide dismutase(SOD), malondialdehyde(MDA), glutathione peroxidase(GSH-Px) in serum and alanine aminotransferase(ALT), oxalic aminotransferase(AST) in liver tissues were detected by micromethod. The levels of inflammatory factors IL-1β, IL-6 and TNF-α in liver tissues were detected by ELISA. The mRNA expression levels of Casp3, Bax, Bcl-2, IκK, IκB and NF-κB in liver tissues were detected by Real-time PCR.

RESULTS  A total of 17, 11, 40, and 68 compounds were identified from Salviae Miltiorrhizae Radix Et Rhizoma, Carthami Flos, Notoginseng Radix et Rhizoma, and compatible sample, respectively. Body weight and organ index(heart, liver, spleen, lung, kidney and pancreas) were not significantly changed in each group. Compared with the Con group, the contents of SOD and GSH-Px were significantly decreased and the contents of MDA and ALT were significantly increased in serum of model group, and the expression levels of IL-1β, IL-6, TNF-α protein and Casp3, IκK, IκB, NF-κB mRNA in liver tissues of model group were significantly increased. Compared with the model group, Carthami Flos and Notoginseng Radix et Rhizoma significantly increased the content of SOD in serum, and the content of SOD and GSH-Px were significantly increased and the content of MDA was significantly decreased in serum of combination group; Notoginseng Radix et Rhizoma, Salviae Miltiorrhizae Radix Et Rhizoma and compatible sample significantly decreased serum ALT content; The expression level of Casp3 mRNA in liver tissues of all groups were significantly decreased, and the expression level of Bcl-2/Bax mRNA in liver tissues of Carthami Flos and combination groups were significantly increased; The expression levels of IL-1β, IL-6, TNF-α protein and mRNA of IκK, IκB and NF-κB in liver tissues of Notoginseng Radix et Rhizoma, Salviae Miltiorrhizae Radix Et Rhizoma and combination groups were significantly decreased. Compared with Salviae Miltiorrhizae Radix Et Rhizoma, Carthami Flos or Notoginseng Radix et Rhizoma groups, the SOD content in serum and Bcl-2/Bax mRNA expression level in liver tissues were significantly increased, and the contents of MDA and ALT in serum and the expression levels of IL-1β, IL-6, TNF-α protein and IκK, IκB, NF-κB mRNA in liver tissue were significantly decreased in combination groups.

CONCLUSION  Salviae Miltiorrhizae Radix Et Rhizoma-Carthami Flos-Notoginseng Radix et Rhizoma combination can synergically improve the oxidative stress induced by D-galactose, and its mechanism may be related to activating the enzyme oxidation system, alleviating oxidative stress injury and inhibiting the activation of inflammatory pathways.