Difference of Phenylethanol Glycosides in Fresh Cistanches Herba from 2 Hosts Based on UPLC-QQQ-MS and PLS-DA Analysis

OBJECTIVE To comparatively evaluate the differences of phenylethanol glycosides content in the juice and residue of fresh Cistanches Herba with the hosts of Atriplex canescens and Haloxylon ammodendron.

METHODS The contents of eight phenylethanoid glycosides in the juice and residue of samples derived from two different host plants were compared using ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QQQ-MS). Furthermore, partial least squares discriminant-analysis(PLS-DA) was employed to identify and evaluate the differences in phenylethanoid glycoside profiles between the obtained from the two host plants.

RESULTS The phenylethanol glycosides contents in the juice and residue of with the host of Atriplex canescens were higher than those in Cistanches Herba with the host of Haloxylon ammodendron. The phenylethanol glycoside content of Atriplex canescens-Cistanches Herba and Haloxylon ammodendron-Cistanches Herba residues, which were enzyme-killed for 5 min and then dried at 80 °C, was 131.5108% and 93.2983% higher than that of the direct-dried without enzyme-killing, respectively.

CONCLUSION Using phenylethanol glycosides, the main components in Cistanches Herba as a judgement index, the differences in active ingredients in the juice and residue of fresh Atriplex canescens-Cistanches Herba and Haloxylon ammodendron-Cistanches Herba can be assessed quickly and efficiently. And the content of phenylethanol glycosides is higher in residues, and killing the enzyme for 5 min can effectively retain the phenylethanol glycosides contents in fresh Cistanche Herba.