Difference of Phenylethanol Glycosides in Table Cistanches Herba from 2 Hosts Based on UPLC-QQQ-MS and PLS-DA Analysis

OBJECTIVE To comparatively evaluate the differences of phenylethanol glycosides in the juice and residue of table Cistanches Herba with the hosts of Atriplex canescens and Haloxylon ammodendron.

METHODS Comparative evaluation of the differences 8 phenylethanol glycoside components in the juice and residue of fresh Cistanches Herba from two hosts by ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QQQ-MS) and combining with partial least squares discriminant-analysis(PLS-DA).

RESULTS The phenylethanol glycosides contents in the juice and residue of fresh Cistanches Herba with the host of A. canescens were higher than those in Cistanches Herba with the host of H. ammodendron. The phenylethanol glycoside content of A. canescens-Cistanches Herba and H. ammodendron-Cistanches Herba residues, which were enzyme-killed for 5 min and then dried at 80 °C, was 131.5165% and 93.2983% higher than that of the direct-dried without enzyme-killing, respectively.

CONCLUSION Using phenylethanol glycosides, the main components in Cistanches Herba as a judgement index, the differences in active ingredients in the juice and residue of fresh Atriplex canescens-Cistanches Herba and Haloxylon ammodendron-Cistanches Herba can be assessed quickly and efficiently. And the content of phenylethanol glycosides is higher in fresh Cistanches Herba residues, and killing the enzyme for 5 min can effectively retain the phenylethanol glycosides contents in fresh Cistanche Herba.