Screening of Anti-cardiomyocyte Apoptosis Components in Descurainiae Semen Lepidii Semen and Study of Mechanism

OBJECTIVE To screen components with ameliorative effects on H₂O₂-induced cardiomyocyte injury from 42 compounds isolated from Descurainiae Semen Lepidii Semen and initially to investigate the mechanism of action of the effective compounds.

METHODS The H9c2 cardiomyocyte injury model was induced by 200 μmol·L⁻¹ H₂O₂, and the cells were treated with 42 compounds from Descurainiae Semen Lepidii Semen for 6 h, then measured cell viability, oxidative stress, apoptosis levels and expression of key proteins of the apoptosis pathway.

RESULTS Ten compounds in Descurainiae Semen Lepidii Semen could effectively enhance cell viability. Among them, Kaempferol-3-O-β-D-glucopyranoside (DS-1), quercetin-3-O-β-D-xylopyranoside (DS-2) and lepidiumoside A (LS-19) were the most effective, and they can reduce the apoptosis rate of cardiomyocyte, enhance T-SOD, GSH-PX levels, decrease LDH, MDA levels, improve oxidative stress, enhance mitochondrial membrane potential level, decrease ROS level, and inhibit apoptosis of cardiomyocytes, reduce the level of Bax/Bcl-2, and inhibit the expression level of the key protein Caspase-3 of the apoptosis pathway.

CONCLUSION DS-1, DS-2 and LS-19 from Descurainiae Semen Lepidii Semen have significant protective effect on H9c2 cardiomyocyte in H₂O₂-induced injury, the mechanism may be related to improve the level of oxidative stress homeostasis the and inhibit the apoptotic pathway.