Study on Quality Standard of Compound Banmao Capsules Based on Multicomponent Analysis

OBJECTIVE  To optimize and improve the quality standard of Compound Banmao Capsules.

METHODS Ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1, astragaloside Ⅳ, acanthopanax senticosus and isofraxidin were identified by thin-layer chromatography(TLC). Limit inspection of cantharidin was detected by GC. Ligustrin, Monoside, Loganin, Scutellarin and Scutellarein were determined simultaneous by HPLC. Cosmosil C₁₈(4.6× 250, 5 μm) column was used with acetonitrile-0.3% phosphoric acid solution as mobile phase in gradient elution at a flow rate of 1.0 mL·min^–1, and the detection wavelength were 224 nm, 240 nm, 335 nm, the column temperature was 40 ℃.

RESULTS Microscopic feature was finded easily. The spots in the TLC were clear with good reproducibility. The linear ranges of ligustrin, monoside, loganin, scutellarin and scutellarein were 0.001896−0.5309 μg, 0.01998−0.5594 μg, 0.02028−0.5677 μg, 0.01946−0.5447 μg and 0.008935−0.2502 μg. The average recoveries were 103.87%, 97.21%, 96.32%, 97.52%, 98.18%.

CONCLUSION The method developed is simple, stable, repeatable, which can be used for the quality of Compound Banmao Capsules more effective and comprehensively.