Study on Quality Standard of Compound Banmao Capsules Based on Multicomponent Analysis

OBJECTIVE  To optimize and improve the quality standard of compound Banmao capsules.

METHODS Ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1 in Ginseng Radix et Rhizoma, astragaloside Ⅳ in Astragali Radix, Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis and isofraxidin were identified by thin-layer chromatography(TLC). Limit inspection of cantharidin was detected by GC. Ligustrin, monoside, loganin, scutellarin and scutellarein were determined simultaneous by HPLC. Cosmosil C₁₈(4.6 mm×250 mm, 5 μm) column was used with acetonitrile-0.3% phosphoric acid solution as mobile phase in gradient elution at a flow rate of 1.0 mL·min^–1, and the detection wavelength were 224, 240, 335 nm, the column temperature was 40 ℃.

RESULTS Microscopic feature was found easily. The spots in the TLC were clear with good reproducibility. The linear ranges of ligustrin, monoside, loganin, scutellarin and scutellarein were 0.001896−0.5309 μg, 0.01998−0.5594 μg, 0.02028−0.5677 μg, 0.01946−0.5447 μg and 0.008935−0.2502 μg. The average recoveries were 103.87%, 97.21%, 96.32%, 97.52%, 98.18%.

CONCLUSION The established method is stable reliable, highly specific, simple, efficient, and more comprehensive control of the overall quality of compound Banmao capsules, which provides reference for its quality evaluation.