OBJECTIVE To explore hypoglycemic effect of 95% ethanol fraction of Nitraria roborowskii Kom(NRK-C) and its possible mechanism evaluated in the type 2 diabetes mellitus(T2DM) mice.
METHODS The body weight, organ indices, blood glucose levels, serum biochemical indexes, as well as HE/PAS histopathological section were all analyzed to assess the hypoglycemic effect of NRK-C in T2DM mice induced by a high-fat diet(HFD) combined with six intraperitoneal injections of 35 mg·kg-1 of streptozotocin(STZ). The Western blotting and immunofluorescence were further applied to determine the regulatory effect of NRK-C on key signaling proteins.
RESULTS The fasting blood glucose levels were significantly reduced after 7 weeks of administration of NRK-C. In addition, NRK-C could also significantly improve glucose tolerance, hepatic glycogen levels, and lipid levels(total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein), and significantly reduced insulin resistance of diabetic mice, which played an important role in the antidiabetic effects. Further mechanism research demonstrated that phosphorylated PI3K expression was up-regulated and p-GSK3β expression was up-regulated after NRK-C intervention, indicating that NRK-C might exert a potential antidiabetic effect by modulating the PI3K/AKT signaling pathway.
CONCLUSION All these results suggested that NRK-C might improve T2DM and had the potential to be used as an adjunctive therapy.
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