Study on Diterpenoid Alkaloids from Aconitum Brachypodum and Their Cardiotoxicity

OBJECTIVE  To study the sturcture and cardiotoxicity of diterpenoid alkaloids from the roots of Aconitum brachypodum.

METHODS Silica gel column chromatography and spectroscopy was used to separate and identify the structures of compounds. The cardiotoxicity of different compounds were compared using a rat model.

RESULTS  Twelve diterpenoid alkaloids were isolated and identified as secokaraconitine(1), oxonitine(2), chasmanine(3), crassicauline A(4), neoline(5), deoxyaconitine(6), aconitine(7), indaconitine(8), yunaconitine(9), talatisamine(10), benzoylaconine(11), and aconine(12). Through cardiotoxicity assay, it had been confirmed that 1-OH, 3-OH, 8-COCH₃, and 14-benzoyl group could increase cardiotoxicity of aconitane compounds, while 6-OCH₃, N-CHO and N=C could reduce it.

CONCLUSION  The effects of different substituents on the cardiotoxicity of aconitane compounds are clarified through overall animal experiments.