OBJECTIVE To identify the chemical constituents in Geranium Strictipes Knuth based on ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS).
METHODS Using ACQUITY UPLC-BEH C18(2.1 mm×100 mm, 1.7 μm) column, using acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. Mass spectrometry conditions: using ESI electron Spray ion source for full scan of positive and negative ions.
RESULTS By analyzing the mass spectrometry data and referring to relevant literature, combined with the comparison results of reference substances, a total of 67 chemical components were identified, including 21 tannins, 20 organic acids, 15 flavonoids and their glycosides, and 11 terpenes.
CONCLUSION The rapid and accurate establishment of this study can provide a reference for the control research and material basis research of Geranium Strictipes Knuth.
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