Preparation of the Baicalein-Zinc Complex Nano-delivery System and Effect on Inflammatory Bowel Disease in Mice

OBJECTIVE To prepare Baicalein-Zinc(Ba-Zn) complex nano-delivery system for the treatment of inflammatory bowel disease(IBD) in mice.

METHODS  The Ba-Zn complex was prepared, and it was modified with chondroitin sulfate(CS) to self-assembly prepare Ba-Zn@CS nano-delivery system. Ba-Zn@CS nanoparticles were characterized by Fourier transform infrared spectrometer(FT-IR) and transmission electron microscope(TEM), and its particle size, potential were determined. The therapeutic effect of the Ba-Zn@CS nano-delivery system was evaluated in the IBD mice from aspects such as the disease activity index(DAI), the length of the colon length, and the pathological analysis of colon tissue pathology.

RESULTS  FT-IR and TEM proved that the successful construction of Ba-Zn@CS nanoparticles with a particle size of 356.4 nm, polydispersity index(PDI) of 0.129, surface zeta potential of −35.7 mV. The in vivo efficacy evaluation showed that the weight loss of the Ba-Zn@CS treatment group was significantly alleviated, the DAI was reduced, and the colon length was longer than the mice of the model group(P<0.05). The pathological examination of colon tissue showed that the mice in the Ba-Zn@CS treatment group had a significant alleviating local inflammation of the colon.

CONCLUSION  The Ba-Zn@CS nano-drug delivery system improve the in vivo anti-IBD effect of free Ba drugs, alleviate local inflammation in IBD mice, and provide a new approach to enhance the bioavailability of Ba for the treatment of IBD.