Identification of Illegal Additive Propoxydescarbon Desmethyl Carbodenafil in Health Food Based on De Novo Synthesis Strategy of Reference Substance

OBJECTIVE  To determine a new carbodenafil derivative illegally added in health foods by the de novo synthesis strategy of reference substance.

METHODS  The samples were extracted by methanol and injected to ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry for non-targeted screening, the accurate molecular weight and secondary fragment ions of the analyte was then obtained. After separation by silica gel column chromatography, the structure of the analyte was determined by nuclear magnetic resonance spectroscopy, and compared with the reference substance from de novo synthesis strategy.

RESULTS  The analyte was identified as propoxydescarbon desmethyl carbodenafil.

CONCLUSION  Propoxydescarbon desmethyl carbodenafil is not included in the additional testing methods and is detected as a novel additive in the dietary supplement for the first time.