Chemical Constituents of Endophytic Fungi Trichoderma sp. SHJN1 and Perenniporia sp. SHJG1 from Lindera glauca and Their Anti-tumor Activities

OBJECTIVE To find lead compounds with potent bioactivities from endophytic fungi Trichoderma sp. SHJN1 and Perenniporia sp. SHJG1 from the traditional Chinese medicine Lindera glauca. METHODS The fermentation of Trichoderma sp. SHJN1 and Perenniporia sp. SHJG1 were isolated and purificated by repeated silica gel, ODS, Sephadex LH-20, and semi-preparation HPLC. The structures of compounds were performed by NMR and ESI-MS. The preliminary evaluation of anti-tumor activity of these compounds was performed by human breast cancer cells(MCF-7) and human lung cancer cells(A549). RESULTS A total of 12 compounds were purified from the above two endophytic fungi and identified as alantrypinone(1), oryzalactam(2), phomoindene A(3), cis-gregatin B(4), huaspenone B(5), stigmasta-7,22-dien-3β,5α,6α-triol(6) and ergosterol(7), 1-deoxy-2-demethylviridiol (8), viridiol(9), trichodermamides A(10), chromone(11) and 4-hydroxyphenylacetic acid(12). The evaluation of antitumor activity showed that, compound 3 showed inhibitory activity on MCF-7 cell line with an IC₅₀ value of (62.9±1.02)μmol·L^-1cisplatin(DDP) IC₅₀=(30.1±1.67)μmol·L^-1, while compound 8 and 9 showed inhibitory activities on A549 cell line with IC₅₀ values of (34.6±1.57)μmol·L^-1 and (44.9±1.74)μmol·L^-1, respectivelyDDP IC₅₀=(20.6±1.42)μmol·L^-1. CONCLUSION Compounds 3, 8 and 9 show potent anti-tumor effects.