Mechanism of Antagonistic Action of Soluble Components of Salvia Miltiorrhiza on Apoptosis of HTR8/SVneo Induced by Lipopolysaccharide

OBJECTIVE To explore the anti-apoptosis mechanism of Salvia miltiorrhiza soluble substances on HTR8/SVneo induced by lipopolysaccharide(LPS). METHODS HTR8/SVneo cells were treated with LPS of different concentrations(0, 100, 200, 400, 800, 1 600 ng·mL^-1), and Real-time quantitative PCR was used to verify the establishment of HTR8/SVneo cell inflammation model. Cell viability in each group was detected by cell counting Kit-8. Cells migration ability to the injured area was evaluated by scratch test. Flow cytometry was used to detect cell apoptosis rate in each group and analyze mitochondrial membrane potential.RESULTS After 200 ng·mL^-1 LPS intervention, the transcriptional level of NLRP3 inflammasome and inflammatory cytokines in HTR8/SVneo cells were increased(P<0.05). Compared with LPS group, the activity of HTR8/SVneo was significantly increased after the intervention of salvianolic acid B(0.08 μmol·L^-1), salvianolic acid C(0.4 μmol·L^-1) and lithospermic acid(0.08 μmol·L^-1)(P<0.01). Compared with LPS group, the apoptosis rate of Salvia miltiorrhiza soluble substance intervention group was significantly decreased(P<0.05 or P<0.01), the level of mitochondrial membrane potential was significantly increased. CONCLUSION Salvia miltiorrhiza soluble substance can enhance the cell viability of HTR8/SVneo induced by LPS, improve cell migration ability, enhance mitochondrial membrane potential, and then inhibit cell apoptosis.