Study on the Effect and Mechanism of Yiwei Decoction in the Treatment of N-methyl-N’-nitro-N-nitrosoguanidine-induced Chronic Atrophic Gastritis Rats and Gastric Cancer Rats

OBJECTIVE  To explore the effect and potential molecular mechanisms of Yiwei Decoction on chronic atrophic gastritis(CAG) and gastric cancer.

METHODS  N-methyl-N’-nitro-N-nitrosoguanidine(MNNG) was used to induce the model of CAG/gastric precancerous lesions and gastric cancer in rats. Therapeutic effect of Yiwei Decoction after intervention was observed. High-throughput sequencing technology and RT-qPCR were used to detect the change of transcriptome in stomach and blood of MNNG-induced tumor rats.

RESULTS  Yiwei Decoction significantly ameliorated the MNNG-induced CAG and intestinal metaplasia in rats, and significantly reduced the weight of tumor at gastroduodenal junction. Induction of MNNG obviously increased the mRNA level of Vcan, BGIG10116_32332 and rno-miR-542-3p in rat stomach and rno-miR-363-3p in rat serum, while Yiwei Decoction reversed these change. Moreover, Yiwei Decoction could slightly reduce the expression of gastric cancer therapeutic targets including VEGFR and HER-2.

CONCLUSION  Yiwei Decoction is effective in the treatment of MNNG-induced CAG and intestinal metaplasia; and it can further inhibit the progression of MNNG induced gastric and duodenal tumors in rats. Its pharmacological mechanism may be achieved by inhibiting the overexpression of Vcan, lncRNA BGIG10116-32332, and rno-miR-542-3p in the stomach, as well as suppressing the overexpression of rno-miR-363-3p in the blood.