Simultaneous Determination of the Contents of 9 Components in Xiao Chengqitang Granules by Quantitative Analysis of Multi-components by Single-marker

OBJECTIVE To establish a quantitative analysis of multi-components by single-marker(QAMS) method for the simultaneous determination of naringin, neohesperidin, aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether, honokiol, magnolol in Xiao Chengqitang granules. METHODS HPLC was used with Waters Xbridge C₁₈ column (250 mm×4.6 mm, 5 μm). The column temperature was 30 ℃. The detection wavelength was 280 nm(naringin, neohesperidin), and 254 nm(aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether, honokiol, magnolol). The mobile phase was acetonitrile-0.1% phosphoric acid solution for gradient elution. The flow rate was 1.0 mL·min^-1. The sample volume was 10 μL. Taking neohesperidin as the internal reference, the relative correction factor of this component and the other 8 components was calculated, and the content of each component was calculated. The QAMS calculation results were compared with the measured results of the external standard method to verify the feasibility of the established method. RESULTS The 9 components had a good linear relationship within their specified concentration ranges(r>0.999), the average recovery rate was 87.06%-98.08%, and the RSD was 1.21%-2.83%. There was no significant difference between the calculated values by QAMS and the measured values by the external standard method. CONCLUSION The established method is accurate and reliable. The QAMS method can be used for the simultaneous determination and quality control of the content of 9 components in Xiao Chengqitang granules.