Study on the Quality Evaluation of Sijisanhuang Tablets Based on Quantitative Analysis of Multi-components by Single Marker

OBJECTIVE To establish a quantitative analysis of multi-components by single marker(QAMS) method for simultaneous determination of baicalin, unmethylated bacicalein, melaeuca A-7-O-β-D-glucuionide, scutellarin, bacicalein, wogonin, melaeuca A in Sijisanhuang tablets. METHODS The separation was performed on HPLC with Shimadzu VP-ODS column(4.6 mm×250 mm, 5 μm), with the mobile phase consisting of acetonitrile-0.1% trifluoroacetic acid elution for gradient elution. The flow rate was 1.0 mL·min^-1, the column temperature was 30 ℃, and the detection wavelengths were 276 nm. The relative correction factors of unmethylated bacicalein, melaeuca A-7-O-β-D-glucuionide, scutellarin, bacicalein, wogonin, melaeuca A were calculated by using the baicalin as the internal standard. At the same time, the external standard method was used to determine the content of the seven components, and the differences between the two methods were compared. RESULTS The linear ranges of seven components were well(r>0.999 8). And the average recoveries were 97.0%-102.1%, RSDs were 1.2%-2.5%, respectively. There were no significant difference in assay results between QAMS and the external standard method. There were obvious differences in the contents of each component among samples from different enterprises. CONCLUSION The QAMS method can reflect the quality of Radix Scutellariae for feeding, and then comprehensively and effectively evaluate the quality of Sijisanhuang tablets, so as to lay a foundation for improving its quality standard.