Mechanism of Guipi Pills in Treatment of Anxiety Disorder Based on UHPLC-Q-Exactive Orbitrap MS and Network Pharmacology

OBJECTIVE To clarify the chemical composition of Guipi pills and to explore its mechanism of action in the treatment of anxiety disorder.METHODS The UHPLC-Q-Exactive Orbitrap MS technology was used to accurately characterize the chemical components in Guipi pills, and the important components, key targets and pathways of Guipi pills in the treatment of anxiety disorder were analyzed by combining network pharmacology and molecular docking.RESULTS A total of 116 compounds belonging to 9 categories were identified, including phenylpropanoids, flavonoids and their glycosides,terpenoids and their glycosides, alkaloids, organic acids, volatile oils, oligosaccharide esters, amino acids and so on. Through in-depth exploration of network pharmacology and molecular docking, 9 important components, 10 core targets and 15 anxiety disorder-related signaling pathways of returning to Guipi pills in the treatment of anxiety disorder were obtained.CONCLUSION It is predicted that Guipi pills may play a role in the treatment of anxiety disorder by regulating the level of neurotransmitters such as 5-HT and GABA, and reducing the expression of inflammatory factors such as HPA and HPG, which fully embodies the characteristics of multi-component, multi-target and multi-pathway synergistic effect in the treatment of anxiety disorder.