Effects of Hydrogen-rich Saline on Intestinal Damage in High-altitude Burned Mice

OBJECTIVE To explore the protective effect and possible mechanism of hydrogen-rich saline on intestinal injury in burned mice under high altitude environment.METHODS Thirty-six female BALB/c mice were randomly divided into sham group, burn model group and hydrogen-rich saline group, all experiments were conducted in a low-pressure oxygen chamber to simulate an altitude of 3000 m. The back of mice in the sham group was simulated with a warm water bath(37 ℃,10 s), and the back of mice in the burn model group and the hydrogen-rich saline group was simulated with a boiling water bath(90 ℃, 10 s) to cause 30% TBSA Ⅲ scald. After burn, mice in the hydrogen-rich saline group was injected with10 mL·kg^-1(0.6 mmol·L^-1), while the sham group and the burn model group were injected with the same amount of saline. The effects of hydrogen-rich saline on the intestinal barrier and intestinal nervous system of burned mice were analyzed by analyzing the pathological of intestinal tissue, the expression of lipopolysaccharide in serum and the expression of zonula occludens-1(ZO-1), Occludin, choline acetyltransferase(ChAT), tyrosine hydroxylase(TH) and neuronal nitric oxide synthase(nNOS) in intestinal. In addition, by analyzing the expression of malondialdehyde(MDA), catalase(CAT) and glutathione peroxidase(GSH-Px) in serum, expression of inflammatory factors of TNF-α, IL-1β and IL-17A and CD3 T lymphocyte infiltration in intestinal, the effects of hydrogen-rich saline on oxidative stress and inflammatory response in mice burned at high altitude were investigated.RESULTS Compared with the sham group, mice in the burn model group showed significant intestinal pathological damage, and the expressions of ZO-1, Occludin, ChAT, TH and nNOS were significantly decreased.Hydrogen-rich saline significantly improved intestinal pathological injury and intestinal mucosal barrier injury in the model group, and also showed a positive effect on the intestinal nervous system. In addition, hydrogen-rich saline could significantly regulate the expressions of MDA, CAT and GSH-Px in serum, reduce the expression of inflammatory cytokines, and reduce the infiltration of CD3 T lymphocytes in the intestinal tissue of burn model mice.CONCLUSION Hydrogen-rich saline can relieve the intestinal pathological damage, repair the intestinal mucosal barrier, and protect the intestinal nervous system of burned mice under high altitude environment, which may be realized by regulating oxidative stress and inflammatory response.