YANG Zonghan, TAO Guanqi, SONG Wenying, ZHANG Qixin, XU Shifang, HUANG Wenkang, YE Yiping. Rapid Identification of Chemical Components in Xitong Preparations by UPLC-Triple-TOF-MS/MS[J]. Chinese Journal of Modern Applied Pharmacy, 2024, 41(6): 777-786. DOI: 10.13748/j.cnki.issn1007-7693.20224293
    Citation: YANG Zonghan, TAO Guanqi, SONG Wenying, ZHANG Qixin, XU Shifang, HUANG Wenkang, YE Yiping. Rapid Identification of Chemical Components in Xitong Preparations by UPLC-Triple-TOF-MS/MS[J]. Chinese Journal of Modern Applied Pharmacy, 2024, 41(6): 777-786. DOI: 10.13748/j.cnki.issn1007-7693.20224293

    Rapid Identification of Chemical Components in Xitong Preparations by UPLC-Triple-TOF-MS/MS

    • OBJECTIVE  To establish a rapid analytical method to identify the chemical components in Xitong preparations for the treatment of rheumatoid arthritis by ultra-high performance liquid chromatography coupled triple time-of-flight mass spectrometry(UPLC-Triple-TOF-MS/MS).
      METHODS  The analysis was performed on an ACQUITY UPLC HSS T3 (2.1 mm×150 mm, 1.8 μm) column with the mobile phase consisting of water containing 0.1% formic acid(A) and acetonitrile containing 0.1% formic acid(B) in gradient mode at a flow rate of 0.3 mL·min−1. Data acquisition was carried out under positive and negative ion modes. The processed data was analyzed by Peakview software and compound database. The chemical components were determined by comparison with standard products, combining with the characteristic fragments in secondary MS, and those in the related literatures.
      RESULTS  Seventy-eight compounds were identified including 20 phenylpropanoids, 33 terpenes, 19 flavonoids, 6 oxylipins, two of which might be new compounds.
      CONCLUSION  The method can be used for rapid identification of chemical compositions in Xitong preparations, which provides basis for quality control and elucidation of phamarcodynamic material basis of Xitong pills and capsules .
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