Study on Characteristic Chromatogram of Mongolian Medicine Rubus Sachalinensis Leveile and Content Determination of 6 Components

OBJECTIVE To establish the characteristic chromatogram of 11 batches of Rubus sachalinensis Leveile from different producing areas and determine the contents of 6 main components. METHODS Used the CAPCELL PAK MGII C₁₈ column(4.6 mm×250 mm, 5 μm), acetonitrile(A)-0.1% formic acid aqueous solution(B) as mobile phase for gradient elution, detection wavelength of 254 nm. Established the characteristic chromatogram of Rubus sachalinensis Leveile from different places. The common peaks were identified by HPLC-Q-Exactive-MS/MS, and the similarity was evaluated, the content of 6 main components was determined. RESULTS A total of 24 common peaks were demarcated and 18 of them were identified. The similarity between the fingerprint of S1-S8 batches of Rubus sachalinensis Leveile and the control fingerprint was >0.900, and there was a significant difference in similarity among the other three batches with distant origins. Through methodological investigation, the six components showed a good linear relationship within certain concentration range. Precision, stability and repeatability met the requirements. The average recoveries were 97.72%-98.94%. The content of characteristic component (ellagic acid) was high, and the origin distribution was uniform, which could be used as the quality control index ingredient. CONCLUSION In this study, HPLC characteristic chromatogram, quantitative determination and analysis method and HPLC-Q-Exactive-MS/MS qualitative identification method of Rubus sachalinensis Leveile are established, which are simple, accurate and reliable, and can provide reference for the quality control of Rubus sachalinensis Leveile.