Analysis of Chemical Constituents in Guizhi Mahuang Geban Decoction by LC-Q-TOF-MS and LC-IT-MS

OBJECTIVE To study chemical constituents of Guizhi Mahuang Geban decoction(GZMHGBT). METHODS To analyze the extract of GZMHGBT by LC-Q-TOF-MS and LC-IT-MS, the analysis was performed on an Agilent Zorbax SB-C₁₈ Rapid Resolution HT column with gradient elution of 0.05% formic acid(A)-acetonitrile(B) at the flow rate of 0.6 mL·min^-1, the column temperature was 30 ℃. The constituents of GZMHGBT were identified according to the accurate relative molecule weight by LC-Q-TOF-MS and multistage MS information by LC-IT-MS. RESULTS A total of 119 constituents of GZMHGBT were identified, including 26 flavonoids, 47 triterpene saponins, 11 monoterpene glycosides, 7 alkaloids, and 28 other compounds. The structures of 25 constituents were confirmed by comparing with the corresponding reference standards. CONCLUSION The constituents in GZMHGBT are basically revealed, and the results can provide reference for the quality evaluation and effective constituents discovery of GZMHGBT.