Dynamic Pharmacodynamic changes and Mechanism of Corni Fructus Before and After Wine-processed in Postmenopausal Osteoporosis Rats based on active components

OBJECTIVE To compare the differences of five active components in Corni Fructus and wine-processed Corni Fructus: 5-hydroxymethyl furfural(5-HMF), iridoid glycosides(monoside, loganin, swertioside), gallic acid, and to explore the dynamic pharmacodynamic changes and mechanism on postmenopausal osteoporosis(PMOP) model rats of Corni Fructus and wine-processed Corni Fructus on 30, 60, 90 d after operation. METHODS The analysis was performed on Hypersil C₁₈chromatography(4.6 mm×250 mm, 5 μm), with the gradient elution of acetonitrile(A)-0.3% phosphoric acid(B) solution at flow rate of 0.6 mL·min^-1. The column temperature was 30 ℃ and the detection wavelength was set at 240 nm (iridoid glycoside), 274 nm (5-HMF, gallic acid). The female SD rats were randomly divided into sham group, model group, low-dose and high-dose of Corni Fructus and wine-processed Corni Fructus groups, and all of them were emasculated and modeled except the sham group. on 30, 60 and 90 d after operation, the dynamic changes of bone mineral density(BMD), serum estradiol(E₂), calcium(Ca), phosphorus(P) and microstructure of bone tissue were measured.RESULTS The total content of five active components, 5-HMF and gallic acid in wine-processed Corni Fructus was higher than that of Corni Fructus. Compared with the model group, the high-dose group of wine-processed Corni Fructus could increase the levels of serum E₂, Ca and P on the 30 and 60 d after operation, the BMD could also be increased on the 60th day after operation(P<0.05). On the 90 d after operation, both the high-dose of Corni Fructus and wine-processed Corni Fructus groups could increase the BMD, serum E₂, Ca and P levels of the model rats(P<0.05), the high-dose wine-processed Corni Fructus group could also reduce the fracture of bone trabeculae and the content of adipocytes in the bone marrow cavity. CONCLUSION Corni Fructus and wine-processed Corni Fructus can alleviate PMOP, high-dose is better; wine-processed Corni Fructus can intervene the occurrence and development of the disease in the early stage. The mechanism may be related to increasing E₂ level, slowing down the loss of Ca and P in bone, regulating bone metabolism and delaying the decline of bone density, the higher 5-HMF and gallic acid than that of Corni Fructus may be one of the main factors for the better efficacy of wine-processed Corni Fructus.