Formulation Process Optimization and Pharmacodynamic Mechanism of Bitter Wine Soup

OBJECTIVE To optimize the processing technology and formula of Bitter Wine Soup, and to explore its efficacy and mechanism. METHODS The formula of Bitter Wine Soup was optimized by a single factor combined with an orthogonal test, and the average diameter of the inhibition zone was used as the evaluation index for formula optimization of Bitter Wine Soup. Prepare control group, model group, positive drug group, Bitter Wine Soup low, medium and high dose groups and Bitter Wine Soup ancient prescription group rat drug-containing serum, cultured human bronchial epithelial cells(16HBE) in vitro, intervened with 20 μg·mL^-1 lipopolysaccharide and 10% drug-containing serum of different groups, the effect of drug-containing serum on lipopolysaccharide-induced 16HBE cell proliferation was detected by CCK-8 method, and the contents of tumor necrosis factor α (TNF-α), interleukin 6(IL-6), interferon-1β(IL-1β) of leukocytes in cell supernatant were detected by ELISA, the expression of P65 and IκBα mRNA was detected by PCR. RESULTS The optimal formula for Bitter Wine Soup was as follows: 60 mL rice vinegar, 30 g egg white, 10 g Pinelliae Rhizoma Praeparatum Cum Alumine, and 1 g Membrana Follicularisovi. Compared with the control group, the proliferation activity of 16HBE in the model group was decreased(P<0.01), the contents of TNF-α, IL-1β and IL-6 were increased(P<0.01), the expression of P65 mRNA was up-regulated, and the expression of IκBα mRNA was down-regulated(P<0.01). Compared with the model group, the cell proliferation activity in the Bitter Wine Soup medium and high dose groups and the ancient prescription group increased(P<0.01), the contents of TNF-α, IL-1β and IL-6 decreased(P<0.01), the expression of P65 mRNA was down-regulated, and the mRNA of IκBα expression was up-regulated(P<0.01). Compared with the Bitter Wine Soup ancient prescription group, the contents of TNF-α and IL-1β in the Bitter Wine Soup high-dose group decreased(P<0.01), and the expression of P65 mRNA was down-regulated(P<0.01).CONCLUSION The optimized formulation and processing technology of Bitter Wine Soup is convenient for preparation, and the dossage is clear. Its drug-containing serum can alleviate lipopolysaccharide-induced 16HBE inflammatory response, and its efficacy is better than that of the original formula. Its mechanism may be related to the inhibition of NF-κB signaling pathway.