Establishment of Two Reference Substances for Determination of Multiple Components for 4 Components in Armeniaca Mume

OBJECTIVE To establish two reference substances for determination of multiple components method to simultaneously determine the content of 4 components of chlorogenic acid, rutin, hyperoside, and isoquercitrin in Armeniaca mume to control and evaluate the quality of Armeniaca mume cost-effectively and efficiently. METHODS HPLC was used. The mobile phase was phase A with 0.1% formic acid in acetonitrile and phase B with 0.1% formic acid solution. The flow rate was 1.0 mL·min^-1, the detection wavelength was 355 nm, and the column temperature was 25 ℃. Chlorogenic acid and hypericin were screened out by DRS Origin software as reference substances, the retention time of chromatographic peaks was predicted by double-standard linear calibration method, and the content of each component was determined by relative correction factor method. RESULTS The prediction results of the double-standard linear correction method in predicting the retention time of different components were accurate and better than the relative retention time method; the relative error between the content calculated by the relative correction factor method and the results of the external standard method was < 1%, and there was no significant difference. The contents of chlorogenic acid, rutin, hyperoside and isoquercitrin in 10 batches of Armeniaca mume were 46.18-69.68, 3.91-8.00, 1.76-3.11, 3.21-5.56 mg·g^-1, respectively. CONCLUSION The established two reference substances for determination of multiple components method is accurate in predicting the retention time of the components to be tested, the chromatographic column has a wide range of applications, saves reference substances, and is economical and efficient; at the same time, the content of each component can be accurately determined, which provides new ideas and methods for the quality control and evaluation of Armeniaca mume.