Establishment and Application of Real-time Fluorescence PCR Detection of Fritillaria Cirrhosae Bulbus in Ying’er Jianpi Powder

OBJECTIVE To explore the feasibility of dual real-time fluorescence PCR in the identification of the authenticity of Fritillaria Cirrhosae Bulbus in Ying'er Jianpi powder. METHODS A dual detection method was established by using specific TaqMan primers and probes to identify Fritillaria Cirrhosae Bulbus and its common counterfeits. Then, its sensitivity, specificity and self-made formula of simulated Ying'er Jianpi powder were evaluated. The method was further used to detect the authenticity of Fritillaria Cirrhosae Bulbus in Ying'er Jianpi powder circulating in the market. RESULTS The established dual real-time fluorescent PCR method had high specificity with a sensitivity of 0.000 1 ng·µL^-1. Six of 49 batches of Ying'er Jianpi powder samples were found to be unqualified, including 2 batches detected no Fritillaria L. and 4 batches detected no Fritillaria Cirrhosae Bulbus. CONLUSION This dual real-time fluorescence PCR method can successfully identify the authenticity of Fritillaria Cirrhosae Bulbus in Ying'er Jianpi powder, and can provide a basis for the identification of Fritillaria Cirrhosae Bulbus in compound preparations.