Metabolite Identification of Morin in Rats by UPLC-Q-Exactive Plus Orbitrap-MS

OBJECTIVE To study the metabolites of morin in rats in vivo by UPLC-Q-Exactive Plus Orbitrap-MS. METHODS Rat plasma, urine and feces samples were collected after oral administration of 20 mg·kg^-1 morin, and the metabolites of morin in vivo were detected by UPLC-Q-Exactive Plus Orbitrap-MS. RESULTS Two glucuronidation metabolites were observed in rat plasma and urines, and one dehydrogenation metabolite was in feces according to the MS and MS/MS information. CONCLUSION The main metabolic pathway of morin is glucuronidation in rats. This preliminary study clarified the metabolism of morin in rats, and provided the basis for further study of pharmacological mechanism.