Study on Anti-peripheral Fatigue Effect of Zhonghua Dieda Pills by Regulating Calcium Channel and ATP Metabolism

OBJECTIVE To establish the zebrafish hypoxia-fatigue model, and to study the anti-peripheral fatigue effect of Zhonghua Dieda pills and its mechanism. METHODS The hypoxia-fatigue model of zebrafish was established by sodium sulfite. The anti-fatigue effects of Zhonghua Dieda pills were observed from six aspects:exercise, content of lactic acid, ATP production, degree of pain, free radical scavenging and calcium ion metabolism. RESULTS Zhonghua Dieda pills(250, 500, 1 000 and 2 000 µg·mL^-1) could significantly improve the exercise ability of fatigued zebrafish, promote lactic acid metabolism, and reduce the content of lactic acid. Zhonghua Dieda pills 1 000 µg·mL^-1 could significantly promote ATP energy synthesis of fatigued zebrafish, while having an analgesic effect. Zhonghua Dieda pills 500, 1 000 and 2 000 µg·mL^-1 could significantly increase the relative expression of SOD1 gene. Zhonghua Dieda pills 1 000, 2 000 µg·mL^-1 could significantly increase the relative expression of CACNA1DA gene. CONCLUSION Zhonghua Dieda pills has obvious anti-peripheral fatigue and analgesic effect, the mechanism maybe related to promoting lactate metabolism, promoting ATP production, increasing the relative expression of SOD1 gene and increasing the relative expression of CACNA1DA gene.