Investigation on Stability and Hospital Dispensing Management of Nysfungin Liniment Prepared with Different Media

OBJECTIVE To established an HPLC method to determine the content of nysfungin liniment, and to investigate the stability of nysfungin liniment prepared with different solvents. METHODS Nysfungin liniment was prepared by 0.9% sodium chloride injection, 2.5% sodium bicarbonate solution, glycerol enema and pure glycerol. Each liniment was divided into two groups and stored at (25.0±0.5)℃ and (5.0±0.5)℃, respectively. The content of nysfungin was determined on day 0, 7, 14, 21 and 28, and the stability of each liniment was investigated. RESULTS The content of nysfungin liniment prepared with 0.9% sodium chloride, glycerine enema and pure glycerol did not change significantly within 4 weeks, and the measured results were (102.2±1.3)% and (102.7±1.3)%, (102.0±1.9)% and (103.1±1.3)%, (99.9±3.3)% and (101.5±2.0)%, respectively. The content of nysfungin liniment prepared by 2.5% sodium bicarbonate changed significantly, the measured results were (85.2±2.1)% and (84.3±1.26)% on day 0 and (59.2±1.7)% and (65.1±2.4)% on day 28. CONCLUSION The content of nysfungin liniment prepared with 0.9% sodium chloride, glycerine enema and pure glycerol don’t change significantly within 4 weeks, and the three media can replace each other when necessary. However, nysfungin in 2.5% sodium bicarbonate was unstable and 2.5% sodium bicarbonate can not be used as a mediator.