Study on Anti-fatigue effect of Superfine Powder of Astragali radix Based on the intestinal microecology

OBJECTIVE To observe the anti-fatigue effect of Astragali Radix superfine powder on C57BL/6 mice and the changes of intestinal flora.METHODS The C57BL/6 mice were divided into Astragali Radix superfine powder high-dose group(2.50 g·kg^-1), medium-dose group(1.25 g·kg^-1), low-dose group(0.63 g·kg^-1), and traditional decoction group(2.5 g·kg^-1), traditional powder group(2.5 g·kg^-1) and blank group. Gavage was given for 30 consecutive days, during and after the administration, the body weight, behavioral indicators and biochemical indicators of the mice were measured. On the 30th day of perfusion, the contents of the colon were aseptically taken, DNA was extracted, the V3+V4 region of the 16S rDNA gene of the intestinal flora was amplified, and the β diversity and α diversity, the phylum and species of intestinal flora were analyzed by high-throughput sequencing.RESULTS Compared with the blank group, the rod climbing time, rotating rod distance and running distance of mice in the Astragali Radix superfine powder high-dose group were prolonged by 14.8%(P<0.05), 26.8%(P<0.01) and 16.2%(P<0.01); mouse liver glycogen and skeletal muscle ATP increased, respectively 20.48% and 20.86%(P<0.01) and the content of BUN and LA decreased by 5.87%(P<0.01) and 12.34%(P<0.01). The activity of T-SOD in serum increased by 18.15%(P<0.01), and the level of MDA was significantly reduced by 16.14%(P<0.01). The top 20 mouse gut microbiota levels were Ruminiclostridium(p=0.001 7) and Lachnoclostridium(p=0.014 1), both increased.CONCLUSION Astragali Radix superfine powder has obvious anti-fatigue effect, which is better than the other two methods of administration, and is directly related to increasing energy supply, anti-oxidation, and reducing the accumulation of metabolites. In addition, the intestinal flora of the Astragali Radix superfine powder group is significantly different from that of the blank group. The change of the intestinal flora may be indirectly related to the anti-fatigue of Astragali Radix superfine powder decoction pieces.