Quality Control of Chlorociprofloxacin in Ciprofloxacin Hydrochloride

OBJECTIVE To establish an HPLC method for determination of chlorociprofloxacin in ciprofloxacin hydrochloride.METHODS The analysis was performed on a Welch Ultimate Plus C₁₈ column (4.6 mm×250 mm,5 μm) with gradient elution.The flow rate was 1.5 mL·min^–1,and the detection wavelength was 278 nm.RESULTS The other impurities and ciprofloxacin was completely separated from chlorociprofloxacin.Chlorociprofloxacin revealed good linearity over the range of 1.050–10.5 μg·mL^–1(r=0.999 9),and the average recovery was 108.8%,while the RSD was 1.97%.CONCLUSION The method is rapid,accurate,sensitive,and can be well control the quality of ciprofloxacin hydrochloride.