Analysis Chemical Constituents in the Stems of She Medicine Dendropanax Dentiger and Establishing HPLC Fingerprint of Its Anti-inflammatory Active Fraction

OBJECTIVE To confirm and analyze the chemical constituents in the stems of Dendropanax dentiger,and establish the HPLC fingerprint of its anti-inflammatory active fraction. METHODS Compounds were isolated and identified by LC-MS,NMR and other analysis instruments.HPLC with Waters Sunfire^TM C₁₈ column (4.6 mm×250 mm,5 μm) was used.Mobile phase consisted acetonitrile-aqueous solution including 0.2% formic acid and 0.2% tetrahydrofuran with gradient elution.Detection wavelength was 256 nm.Column temperature was 30℃.Flow rate was 1.0 mL·mim^–1 and analytic time was 80 min.Injection volume was 5 μL.RESULTS Ten compounds were isolated and identified as chlorogenic acid (1),3,4-O-caffeoylquinic acid methyl ester (2),3,5-O-caffeoylquinic acid methyl ester (3),4,5-O-caffeoylquinic acid methyl ester (4),saikolignanoside A (5),rutin (6),kaempferol-3-O-rutinoside (7),syringin (8),coniferol (9),sinapaldehyde glucoside (10).Under the same fingerprint chromatogram condition,the methodological results were satisfactory to 12 batches of the anti-inflammatory active fractions.And the similarity range was 0.901-0.992.CONCLUSION The stems of Dendropanax dentiger mainly contain phenolic acids,flavonoids and phenylpropanoids.The established fingerprint can not only reflect the chemical basis of anti-inflammatory of the She medicine,but the identification method also is simple and reproducible.It can provide a scientific basis for the quality evaluation and the confirmation of main anti-inflammatory active components of the stems of Dendropanax dentiger.