Simultaneous Determination of Six Components in Mongolian Medicine Jiruhe-6 by Quantitative Analysis of Multi-components by Single-marker

OBJECTIVE To determine the ellagic acid,cinnamic acid,eugenol,costunolide,dehydrocostus lactone and dehydrodiisoeugenol in Mongolian medicine Jiruhe-6 by quantitative analysis multi-components by single marker (QAMS).And to verify the feasibility and scientificity of this method in the quality control study of Mongolian medicine Jiruhe-6.METHODS QAMS method was used.The test sample was extracted with 75% methanol under reflux.Relative calibration factors (ƒ_k/m) of other 5 components were determined with eugenol as internal standard substance.QAMS and external standard method were used for content calculation and comparison.RESULTS There was a good linear relationship between ellagic acid,cinnamic acid,eugenol,costunolide,dehydrocostus lactone and dehydrodiisoeugenol in their respective ranges (r>0.999 9).The average recovery was 94.80%-98.85%,RSD<2.0%.The relative error between external standard method and QAMS was<2%.CONCLUSION QAMS method is simple,accurate,feasible and reproducible.It can be used for the quality standard control of Mongolian medicine Jiruhe-6.